This study aims to assess the phenotype of the enteric nervous system (enteric neurons) in healthy subjects. The enteric nervous system (ENS) is composed by enteric neurons and enteric glial cells. There is a cross-talk between ENS and the intestinal epithelial barrier (IEB). ENS and IEB together constitute the neuronal-glial-epithelial unit. This unit has a key role in gut functions. The characteristics and the phenotype of the ENS also change according to age and environmental factors. Similar study is ongoing for patients with Spina Bifida. Anorectal data and phenotype of the neuronal-glial-epithelial unit in healthy subjects is required to assess abnormality of these items in patients with neurological disease including Spina Bifida.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
OTHER
Masking
NONE
Enrollment
16
colonic biopsies during endoscopy and anal manometry with barostat and anal endoflip
Rennes University Hospital
Rennes, France
Number of enteric neurons per ganglion
colonic biopsies will be used to study intestinal permeability, colonic inflammation and enteric nervous system
Time frame: data collected at the day of endoscopy
density and activity of the enteric glial cells
colonic biopsies will be used to study intestinal permeability, colonic inflammation and enteric nervous system
Time frame: data collected at the day of endoscopy
rectal adaptation
rectal adaptation is defined by a volume variation during ascending pressure (phasic distension). It will be performed with a bag connected to electronic barostat
Time frame: data collected at the day of endoscopy
rectal perception
It will performed by using 6-point Likert scale ranging from 0 (no sensation) to 6 (intolerable pain)
Time frame: data collected at the day of endoscopy
anal distensiblity
It will be performed by using ENDOFLIP
Time frame: data collected at the day of endoscopy
intestinal permeability
Biopsy samples collected at the time of colonoscopy will be use within a Ussing Chamber able to assess intestinal permeability. The Ussing chamber is used to measure the short-circuit current as an indicator of net ion transport taking place across an epithelium, such as gut mucosa. The measure will be performed on 2 biopsies from each patient.
Time frame: data collected at the day of endoscopy
macroscopic and microscopic colonic inflammation
Colonic inflammation will be assessed during the scope procedure. The physician will assess the normality of the mucosa or not in case of mucosal erythema, ulceration or a decrease of the vascular pattern. Microscopic inflammation will be assessed by the pathologist according to neutrophils infiltration in the gut, crypt abscess, crypt distortion.
Time frame: data collected at the day of endoscopy
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