Building on the findings from the investigators previous study, COMIT I, the purpose of the COMIT II study is to supplement the DEXA measurement of body composition with a supplementary DEXA measurement of visceral adipose tissue and to specifically target the impact of oleic acid consumption on body composition as the primary objective. COMIT II also will include analysis of fatty acid ethanolamines (FAEs) and their precursors to elucidate the mechanisms by which canola oil may be modifying body composition, measurement of endothelial function, inflammatory, adiposity and insulin sensitivity biomarkers, and genetic analyses.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
TRIPLE
Enrollment
28
Regular canola oil will be iso-calorically incorporated into fruit smoothies made with milk and consumed twice daily.
High oleic acid canola oil will be iso-calorically incorporated into fruit smoothies made with milk and consumed twice daily.
A typical "Western diet" fat intake as a control treatment, comprised of 11% MUFA, 11% PUFA (9% omega-6 fatty acids and 2% omega-3 fatty acids), and 13% SFA, will be iso-calorically incorporated into fruit smoothies made with milk and consumed twice daily
Penn State University
University Park, Pennsylvania, United States
Body composition
Will be measured using DXA
Time frame: 1 year 6 months
Plasma lipids
Time frame: 1 year 6 months
Flow mediated dilation (FMD) for endothelial function
Time frame: 1 year 6 months
Lipoprotein subclasses
Time frame: 1 year 6 months
Inflammatory markers
Time frame: 1 year 6 months
Lipid Peroxidation
Time frame: 1 year 6 months
Assessment of fatty acid inter-conversion and synthesis using stable isotope tracers
Time frame: 1 year 6 months
Genetic analysis to evaluate the association between FADS 1 and FADS 2 mRNA and protein expression in peripheral blood mononuclear blood cells (PBMCs)
Time frame: 1 year 6 months
Physical activity level
Will be assessed using an Actigraph GT3X+ activity monitor to be worn for 1 week at the end of each treatment period
Time frame: 1 year 6 months
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