Mediterranean Diet, Trimethylamine N-oxide (TMAO) and Anti-oxidants in Healthy Adults

Federico II University50 enrolled

Overview

Trimethylamine N-oxide (TMAO) is a small organic compound naturally present in fish and seefood or generated by the bacterial breakdown of dietary phosphatidylcholine and carnitine-containing foods within the human gut microbiome. Elevated serum TMAO was previously reported to be associated with an elevated risk for cardiovascular events. Aim of this study was to investigate the association between plasma levels of TMAO with the Mediterranean diet in a cohort of healthy adults.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Mediterranean Diet Trimethylamine N-oxide Cardiovascular Risk Factor

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: healthy subjects of both gender Exclusion Criteria: * age\>65 years * \> BMI 30 kg/m2 * vegetarians * smokers * gastrointestinal diseases or complaints * chronic illnesses or other metabolic diseases (including trimethylaminuria) * abnormal blood chemistry values indicative of organ dysfunctions * nutritional supplements, antibiotics or probiotics within 2 months of recruitment

Outcomes

Primary Outcomes

Adherence to Mediterranean Diet by Predimed Questionnaire

Validated 14-item tool of adherence to the Mediterranean diet, with three categories of adherence (≤5, low adherence, 6-9, average adherence and ≥10 points, high adherence)

Time frame: September 2016 to December 2016

Trimethylamine N-oxide plasma levels

ultra high-performance liquid chromatography

Time frame: September 2016 to December 2016

Secondary Outcomes

cardiovascular risk

metabolic syndrome parameters; Homa Index; Framingham score; ASCVD Risk Estimator

Time frame: September 2016 to December 2016