Infection and cancer is a major cause of death and morbidity, and may be preventable through vaccination. It is not fully understood at the molecular level why some people respond better than others to vaccines until now the technology to assess this has not been available. This has impaired vaccine development. The overall goal of the Human Vaccines Project is to understand the 'rules' of how vaccines work. In this demonstration project the investigators will vaccinate healthy adults with hepatitis B vaccine to start to understand better how it works, ultimately helping with rational vaccine design in the future.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
16
1.0 ml (20 micrograms) suspension of hepatitis B surface antigen for intramuscular injection
Vaccine Evaluation Center
Vancouver, British Columbia, Canada
Antibody response to the first dose of hepatitis B vaccine
Anti-HBs antibody level
Time frame: 28 days post-vaccination following the first dose of vaccine
Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to cellular immune response
Immunophenotyping by flow cytometric analysis of immune cell populations, including antigen-specific T-cell and B-cell responses, and response of immune cells to various stimuli in vitro
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination
Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to transcriptomic response
Gene expression by RNA sequencing of whole blood and single immune cells
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination
Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to proteomic response
Proteomic analysis of plasma and white blood cells
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination
Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to metabalomic response
Metabolomic analysis of plasma
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination
Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to epigenetic response
Epigenetic changes in genome
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination
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Kinetics of the immune response to the first dose of hepatitis B vaccine with respect to lymph node response
Immune responses in local lymph node, and comparison with peripheral blood responses
Time frame: Pre-vaccine and 14 days following first dose only
Identify the DNA sequence of B- and T- cell receptors following vaccination
DNA sequencing of T- and B- cells
Time frame: Baseline (pre-vaccine) and on days 1, 3, 7 and 14 post-vaccination