The aim of the study is to evaluate a physiological concentration of adipose tissue-derived stem cells (ASC) in excised fat versus fat harvested by liposuction. Participants will act as their own control as both extraction methods are performed on each participant.
Adipose tissue-derived stem cells are of great interest due to their relevance in regenerative medicine and tissue engineering. A reason, being their capacity for multipotent differentiation, tissue repair and immune modulation. To evaluate a physiological concentration level of ASCs in human fat we investigate two extraction methods of fat, excision and liposuction. This is done through a standardised setup with patients acting their own control. The study is carried out in accordance with the guidelines provided by the International Federation for Adipose Therapeutics and Science and International Society for Cellular Therapy.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
Fat harvest by liposuction (Colemans method)
Stem cell yield
Stem cell yield measured by antibody stained flow cytometry.
Time frame: At baseline right after isolation.
Stem cell yield
Stem cell yield measured by colony forming units and number of colonies \>50 cells are counted.
Time frame: Cells are cultured from baseline to day 14.
Growth kinetics
Growth kinetics measured by culturing. The population doubling time is determined using log phase of growth
Time frame: Cultured for 7 days and counted.
Adipogenic, chondrogenic and osteogenic differentiation.
Differentiation assays for adipogenic, chondrogenic and osteogenic differentiation with Oil Red O staining, Safranin O staining and Alizarin red S staining.
Time frame: Through study completion, an average of 1 year.
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