This study investigates the effect of genetic variations after supplementation with Brazil nuts in healthy Brazilians. Briefly, all the participants will consume one nut a day for 2 months and will stop the intake for more 2 months. Five blood sampling collection will be performed in one month interval, starting at baseline and ending at 2 months without intervention.
Brazil nuts are the richest source of selenium in nature, a micronutrient that has essential functions in human physiology. Selenium status can be measured by the quantification of biomarkers in the blood. Previous studies have shown that genetic polymorphisms can affect Se status and modulate risk for chronic diseases. In this study, five biomarkers will be quantified in the blood, before and after supplementation with Brazil nuts. The genetic variants will be used to stratify the biomarkers and multivariate linear regressions will be used to explore the influence of the polymorphisms on concentrations of the biomarkers .
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
129
one nut a day for 2 months, no intervention for 2 months
change in Plasma Se concentrations
measured by ICP-MS (ug/L)
Time frame: 4 weeks
change in Plasma Glutathione Peroxidase 3 activity
measured by kinetic assay in a spectrometer (U/L)
Time frame: 4 weeks
change in Plasma Selenoprotein P concentrations
measured by ELISA (mg/dL)
Time frame: 4 weeks
change in Erythrocyte Se concentrations
measured by ICP-MS (ug/L)
Time frame: 4 weeks
change in Erythrocyte Glutathione Peroxidase 1 activity
measured by kinetic assay in an automatic spectrometer (U/gHb)
Time frame: 4 weeks
change in total cholesterol concentration in serum
measured in an automatic spectrometer (mg/dL)
Time frame: 4 weeks
change in tryglicerides concentration in serum
measured in an automatic spectrometer (mg/dL)
Time frame: 4 weeks
change in HDL concentration in serum
measured in an automatic spectrometer (mg/dL)
Time frame: 4 weeks
change in LDL concentration in serum
measured in an automatic spectrometer (mg/dL)
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Time frame: 4 weeks
change in protein-c reactive concentration in serum
measured in an automatic spectrometer (mg/dL)
Time frame: 4 weeks
change in Plasma MDA concentrations (malondialdehyde)
measured in an automatic spectrometer (mg/dL)
Time frame: 4 weeks