Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses: 1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ; 2. The unbalance is therapeutically modified through physical training ; 3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program. This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.
Specific objectives: Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot. The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
TREATMENT
Masking
NONE
Enrollment
14
Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.
Camila Fernanda Cunha Brandão
Ribeirão Preto, São Paulo, Brazil
Changes Body weight
Body weight was measured by digital balance before and after the intervention
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Body composition
The change in body composition through deuterium oxide was evaluated.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes White adipose tissue biopsy
A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3).
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Indirect calorimetry
With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates).
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in fatty acids
Collected in lithium heparin tubes, they were centrifuged.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes oxidative stress
Collected in lithium heparin tubes, they were centrifuged.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes inflammatory cytokines
Collected in lithium heparin tubes, they were centrifuged.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in total cholesterol
Collected in lithium heparin tubes, they were centrifuged.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Physical Performance
Based on the Shuttle Walking Test adaptation.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes in Determination of Lactate
Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Food intake
Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Nitrogen Balance
Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen.
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
Changes Telomere length
peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR).
Time frame: Two times: (1) First day and (2) 10 weeks after adaptation and intervention
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