Protein Ingestion Before Training Low

University of Aarhus9 enrolled

Overview

A randomized interventions study using a crossover design. Ten well trained triathletes or bikeriders will be included for to undergo two trail separated by about 3 weeks. The night before the trail each subject will performe a high intense interval trainings session on an ergometer bike. during the night an infusion of aminoacid tracers will be initiated. The subject is woken at 6.30 a.m. Blood samples and a musclebiopsy will be collected prior to the morning bike ride at 8.00a.m. Immediately before the morning bike ride is started the subject will ingest a drink of 0,5g protein or a non-caloric placebo (random order). During the bike ride and the following 4,5 hours blood will be drawn with regular intervals. at time point 0, 60 and 180 min after the training session biopsies are collected.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

HEALTH_SERVICES_RESEARCH

Masking

SINGLE

Enrollment

Conditions

Protein Metabolism

Interventions

Eligibility

Sex: MALEMin age: 18 YearsMax age: 50 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy well-trained triathletes or cyclists * VO2max \> 55 ml O2/kg/min * Training and has been training more than 7 times a week the last 6 months. Exclusion Criteria: * VO2max \< 55 ml O2/kg/min * Has chronic disease * has incjected with tracer on a previous occasion.

Outcomes

Primary Outcomes

Muscle protein fractional synthetic rate (FSR)

Time frame: FSR was measure over at time period of six hours

Secondary Outcomes

Tracer kinetics dilution model

A-V tracer dilution model was applied across the forearm.

Time frame: six hours

Muscle signalling

Muscle signalling will be determined using western blotting in each muscle biopsy

Time frame: Determined in biopsies sampled before and immediatly after 90min exercise in addition to one and four hours after exercise.

Nitrogen excretion

nitrogen excretion is determined by Plasma and urinary carbide analysis as well as plasma NH4+.

Time frame: Urine is collected during two periods. Collection period one: 12 hours prior to morning exercise. Collection period 2: During and four hours following exercise. Blood samples is drawn repeatedly from before to four hours after exercise.