Blood samples from urticaria patients will be analyzed for viral particles
After digestion with DNase to eliminate human DNA, RNA will be reversed-transcribed and amplified with random primer/ Amplification products will be pooled and sequenced/ After substruction of sequences of vertebrates and highly repetitive sequences, contiguous sequences will be assembled and compared with motifs represented in databases of viruses. which will enable identification of putative protein sequences consistent with viruses
Study Type
OBSERVATIONAL
Enrollment
10
Emek Medical Center
Afula, Israel
viral particles
presence of viral particels
Time frame: 6 months
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