Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.
Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
100
collect nasal polyps from patients
collect inferior turbinates from patients as control arm
ThirdSunYatSen
Guangzhou, Guangdong, China
RECRUITINGlocation of YAP in tissues
through Immunohistochemistry
Time frame: through study completion, an average of 2 years
protein expression level of YAP in tissues
through western blot
Time frame: through study completion, an average of 2 years
expression level of Th1 cytokines in the tissues
through Flow cytometry
Time frame: through study completion, an average of 2 years
expression level of Th17 cytokines in the tissues
through Flow cytometry
Time frame: through study completion, an average of 2 years
activation of hippo-YAP in NSECs on protein aspect
Western Blot
Time frame: through study completion, an average of 2 years
activation of hippo-YAP in NSECs
Flow cytometry
Time frame: through study completion, an average of 2 years
activation of hippo-YAP in NSECs on gene aspect
quantitive real time PCR
Time frame: through study completion, an average of 2 years
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