Safety and Efficacy Study of Islets Xenotransplantation

Inclusion Criteria: * Male and female patients age 18 to 40 years of age; * Type 1 diabetes mellitus for at least 5 years; * Body weight 40 to 60kg; * Ability to provide written informed consent; * Manifest signs and symptoms that are severe enough to be incapacitating; * Patients with poor diabetes control (HbA1c \> 9% ); * Patients have diabetes ketoacidosis or hypoglycemia more than once Exclusion Criteria: * Age \< 18 years or \> 60 years; * Diabetes history \< 5 years; * Body weight \> 80 kg; * Exogenous insulin requirement \> 1 unit/kg/day; * Blood test: hemoglobin male \< 90g/l, female \< 90 g/l) or Wbc \<3×109/L, lymphocyte \<1.5×10\^9/L; or platelet \< 80×10\^9/L; activated- partial-thromboplastin-time （APTT） \> normal value (31-43s)10s; * Liver dysfunction; * Kidney dysfunction; * Cardiopulmonary dysfunction; * Combined mental illness, cancer, infection, severe trauma, pancreatitis, surgery or other stress situations for patients; gastric ulcer patients, any bleeding disorders, tuberculosis infection and active infection including hepatitis B, hepatitis C, HIV, or John Cunningham virus; * Serological detection Epstein-Barr virus (EBV) or Cytomegalovirus (CMV)negative; * Patient have taken immunosuppressant in recent one year With high incidence of malignancy in three generation; * Women not using effective contraception of childbearing age, or planning pregnancy in nearly two years or being pregnant or lactating; * Patients can not comply with the research program to complete the diagnosis and treatment; * Patients not be passed by Ethics committee