Clinical and Genomic Registry of MDS in Asia

Overview

Myelodysplastic syndrome (MDS) is a group of clonal haematopoietic stem cell disorders characterized by ineffective haematopoiesis leading to cytopenia, with a significant risk of progression to acute myeloid leukaemia (AML). Progression to AML and resistance to hypomethylating agents (HMA) are important unmet clinical needs. The pathophysiology of MDS and its progression to AML involve cytogenetic, genetic and epigenetic aberrations, and hence better understanding of the molecular landscape of MDS has important clinical implications. Also, future treatment strategies for MDS may involve exploitation of genetic information in designing more effective therapy encompassing single agents or combinatorial approaches. The proposed cohort study aims to establish a registry of clinical and genomic registry of MDS and secondary AML in Asian patients, which allows the establishment of the mutational profile of patients and prognostic model for survival, as well as exploration of treatment strategies and prediction for treatment response.

Myelodysplastic syndrome (MDS) is a group of clonal haematopoietic stem cell disorders characterized by ineffective haematopoiesis leading to cytopenia, with a significant risk of progression to acute myeloid leukaemia (AML). Conventional prognostic scoring of MDS is based on the degree of cytopenia, the percentage of bone marrow blast infiltration and karyotypic abnormalities. Risk categories based on prognostic scoring determine the therapeutic approaches. Treatment of high-risk MDS involves the use of hypomethylating agents (HMA), and allogeneic haematopoietic stem cell transplantation (HSCT) in younger patients. Clinical studies with HMAs including azacitidine and decitabine have shown a response rate of about 40% in high-risk patients, and median duration of response of merely 9 to 15 months. HMA failure is associated with a dismal outcome and a median survival of less than 5 months. Therefore, progression to AML and resistance to HMA are important unmet clinical needs. The pathophysiology of MDS and its progression to AML involve cytogenetic, genetic and epigenetic aberrations. Genome-wide and targeted analyses from next-generation sequencing have identified mutations that may have prognostic and therapeutic significance. Recurrent mutations in more than 45 genes are found in over 85% of cases. Theses mutations are found in genes involved in DNA methylation (DNMT3A, TET2, IDH1/2), post-translational chromatin modification (EZH2, ASXL1), transcription regulation (TP53, RUNX1, GATA2), the RNA spliceosome machinery (SF3B1, U2AF1, SRSF2, ZRSR2), cohesion complexes (STAG2), and signal transduction (JAK2, KRAS, CBL). Mutations in TP53, EZH2, ETV6, RUNX1, SRSF2 and ASXL1 portend inferior survivals. Specific mutations, such as internal tandem duplications of FLT3 (FLT3-ITD), have been observed during disease progression and are potential therapeutic targets. Data arising from whole-genome sequencing (WGS) have shown that the clonal evolution of MDS to AML is dynamic and complex. The selection of clones during transformation is shaped by acquisition of genetic alterations during clonal expansion, as well as exposure to genotoxic chemotherapy. Better understanding of the molecular landscape of MDS has important clinical implications. Firstly, prognosticating MDS based on molecular aberrations will supplement current models in stratifying patients for treatment. Secondly, molecular markers may better predict response and resistance to treatment with HMAs. Thirdly, detection of targetable molecular markers during treatment resistance or leukaemic transformation may provide an opportunity for specific therapy, as exemplified by the use of FLT3 inhibitors in FLT3-ITD positive secondary AML. Hence, future treatment strategies for MDS may involve exploitation of genetic information in designing more effective therapy encompassing single agents or combinatorial approaches. There are important gaps in knowledge in the field of MDS. First, there is currently no well-established model integrating molecular with clinicopathologic features in prognostic stratification. There is lack of large registry clinicopathologic and molecular information in Asian patients with MDS. To-date, there is paucity of data focusing on the impact of molecular aberrations on prognosis and treatment response. The proposed cohort study aims to establish a registry of clinical and genomic registry of MDS and secondary AML in Asian patients, which allows the establishment of the mutational profile of patients and prognostic model for survival, as well as exploration of treatment strategies and prediction for treatment response.