Ciliation and Mucus Rheology Parameters Determined Via Air-liquid-interface Cell Cultures in Non-smoking, Smoking, COPD and Asthmatic Patients

University Hospital, Montpellier30 enrolled

Overview

This is a prospective study comparing 4 groups: (1) non-smoking controls, (2) smokers without chronic obstructive pulmonary disease (COPD), (3) smokers with COPD, (4) severe asthma. Bronchial biopsy specimens from each subject will be obtained to produce air-liquid-interface cell cultures. These will then be used to make observations concerning cilia and mucus rheology. This is a first pilot study. The working hypothesis is that the largest group differences will be found for cilia densities; the latter metric was thus chosen as a primary criterion.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

DIAGNOSTIC

Masking

NONE

Enrollment

Conditions

Pulmonary Disease, Chronic Obstructive Asthma Smoking

Interventions

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion criteria for non smoking controls: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * The subject has never smoked * No obstructive breathing disorder (forced expiratory volume at one second (FEV1) \>70% of predicted value; the ratio of FEV1 to forced vital capacity (FEV1/FVC) is \> 0.7) * No known respiratory disease Inclusion criteria for smokers without COPD: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Smoker or former smoker (\>= 30 pack years) * No obstructive breathing disorder (forced expiratory volume at one second (FEV1) \>70% of predicted value; the ratio of FEV1 to forced vital capacity (FEV1/FVC) is \> 0.7) Inclusion criteria for smokers with COPD: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Smoker or former smoker (\>= 30 pack years) * Subject presenting with COPD: FEV1/FVC \< 0.7 (based on spirometry values in the medical file and performed in the 3 months prior to inclusion) * The subject requires bronchial fibroscopy (decision made by pneumologist) * COPD of grade "2" or "3" according to GOLD criteria (Global Initiative for Chronic Obstructive Lung Disease) Inclusion criteria for subjects with severe asthma: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Non-smoker or former smoker (\<10 pack years) * Subject presenting with severe asthma: FEV1/FVC \>= 0.7 and FEV1 \< 80% * The subjects requires bronchial fibroscopy (decision made by pneumologist) Exclusion criteria: * The subject has an extensive neoplastic disease * The subject has another active lung disease (tuberculosis, pulmonary interstitium disease, active or recent pulmonary infection) * The subject has had a recent psychiatric disorder (within one year prior to inclusion and documented by a specialist consultation) * Emergency situations (object extractions...) * Subject consuming illicit drugs or alcohol * The subject is in an exclusion period determined by a previous study * The subject is under judicial protection, or is an adult under any kind of guardianship * It is impossible to correctly inform the patient * The subject cannot fluently read French