The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.
Study design Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group \[Healthy individuals\]: 25 Males/Females, aged 25 to 35 years and test group \[Chronic periodontitis patients\]: 25 Males/Females aged 30 to 45 years. Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
50
mononuclear cells from the peripheral blood of chronic periodontitis patients were intervened with PRF, BCP and PRF/BCP combination
probing pocket depth
probing pocket dept was measured using Williams periodontal probe at base line
Time frame: base line
Protein expression on osteoclast cells was estimated with BCP, PRF alone and BCP/PRF combination.
Protein expression on cells was analyzed using Western blot in all the groups
Time frame: 24 hours from baseline
The activity of caspase-3 in OCs.
The activity of caspase-3 was seen in OCs in all the groups
Time frame: 24 hours from base line
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