Protozoans in Biofilm From Patients With Periodontitis

Central Hospital, Nancy, France231 enrolled

Overview

The buccal cavity is colonized by numerous microorganisms whose the number and composition could be modified with medical background (diseases and drugs) and the level of oral hygiene of the patients. Among all microorganisms identified in the periodontium, few of them are implicated in the etiopathogenesis of periodontal pathologies. To date, four major bacteria are identified for their ability to degrade periodontal tissues. Although the periodontitis is established to be the consequence of bacterial virulence and immune dysfunction, these factors fail to explain the refractory periodontitis of some patients to etiologic treatment . Others microorganisms such as protozoans could have an impact on this disease.

Study Type

OBSERVATIONAL

Enrollment

231

Conditions

Periodontitis, Adult

Interventions

non applicableOTHER

non applicable

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion Criteria: * Adults \> 18 years-old * consulting in periodontal department of CHRU * Patients with moderate to severe periodontitis * At least 2 periodontal sites with PPD ≥ 5mm and one healthy site * No scaling root planing in the last 6 months * Patients insured under the Franch social security system Exclusion Criteria: * Pregnants patients * Patients with antibiotic therapy and/ or all medecines which could modify the buccal microbiota in the last 6 months * Tooth with endodontics periapical lesion * Patients having a scaling root planing in the last 6 months * Patients with guardianship

Outcomes

Primary Outcomes

presence of protozoans

identification of protozoans by molecular technique (PCR) -

Time frame: 6 months after the last inclusion: DNA samples were frozen and identified later.

Secondary Outcomes

Periodontal pocket depth (PPD in mm)

Measure of PPD thanks to periodontal probe

Time frame: One day: measure realised during examination of the patient for periodontal treatment.

Clinical attachment Loss (CAL in mm)

Measure of CAL with periodontal probe

Time frame: One day: measure realied during clincal examination of patient.

Plaque and gingival index

Measure of plaque and gingival index (Loe and Silness, Silness and Loe)

Time frame: One day: measure of index during the clinical examination of patient.

Mobility of tooth

Measure of tooth mobility: Yes or not

Time frame: One day: measure of tooth mobility during the clinical examination of patient.

Bleeding On Probing (BOP)

Measure of BOP : Yes or not

Time frame: One day: measure of BOP during the clinical examination of patient.