The study evaluates the effect of nutrient antigens or therapeutic agents on human small intestinal organoids.
Small intestinal biopsies will be taken to establish human intestinal organoids in vitro. The organoids will be propagated and various antigens will be checked for their effects on proliferation, production of inflammatory components or signal cascade.
Study Type
OBSERVATIONAL
Enrollment
375
Department of Medicine 1, Hector Center for Nutrition, Exercise and Sports, Friedrich-Alexander-University Erlangen-Nuremberg
Erlangen, Germany
Determination of proliferation, apoptosis, histology, and cytokine Expression before and after Antigen provocation of organoids with gluten peptides, nutritional Antigens, Glucagon like peptide antagonist, or Tumor nekrose factor antagonists
Determination of effects on organoids after antigen Stimulation with ELISA, histochemistry, RNA or proteome analysis
Time frame: baseline vs 24h vs 48h vs 72h
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