Compare the HMO composition in breast milk of infants with allergic manifestation (atopic dermatitis and food allergy) in the first 3 years of life, healthy infants with allergic mother and healthy infants with non-allergic mother.
* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis
Study Type
OBSERVATIONAL
Enrollment
150
* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis Method of human milk oligosaccharide analysis : 1. Ultra High Pressure Liquid Chromatography(UHPLC) with fluorescence method 2. High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) method
Chulalongkorn University
Pathum Wan, Bangkok, Thailand
RECRUITINGAtopic dermatitis
Relapsing eczematous typical morphology on typical distribution (Scalp, face, neck, and extensor surfaces of the skin) Typical morphology Acute and subacute : erythematous papules and plaques, may vesiculate and become exudative Chronic : lichenification, papules and excoriations
Time frame: first 2 years of life
IgE mediated food allergy
confirmed by oral food challenge
Time frame: first 2 years of life
Non-IgE mediated food allergy
Presentation of clinical ex. bloody stool when repeating episode occurred with the same food more than twice. Improvement of symptom after eliminating of suspected food
Time frame: first 2 years of life
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