This study aims to characterize the neurological basis of obesity and response to surgical and medical treatment by inducing adult pluripotent stem cells into neuronal cells from subjects that have demonstrated extreme response to bariatric surgery or pharmacological treatment for obesity.
The investigators will consent subjects who have achieved extreme response to either bariatric surgery or pharmacologic treatment for obesity and harvest fibroblasts from skin biopsies, which will be reprogrammed to induced pluripotent stem cells (iPSC). These iPSC's will then be differentiated into neural progenitor cells, neurons, astrocytes, and microglia to identify genetic and epigenetic pathways altered in disease-specific neural progenitor cells of the brain.
Study Type
OBSERVATIONAL
Enrollment
6
Sleeve gastrectomy or Roux-en-Y Gastric Bypass
Response to FDA approved anti obesity medications
University of California San Diego
La Jolla, California, United States
DNA sequencing
Perform DNA sequencing from skin biopsy progenitor cells
Time frame: 4 months
Generate human cell based models of obesity
Fibroblasts will be expanded in culture and then reprogrammed to hiPSCs.
Time frame: 4 months
Differentiation to human CNS cells
Disease specific hiPSCs cells will be differentiated into neural progenitor cells, neurons, astrocytes, and microglia
Time frame: 4 months
Identification of genetic and epigenetic pathways
Identify genetic and epigenetic pathways altered in disease-specific neural progenitor cells, neurons, and non-neuronal cells of the brain
Time frame: 4 months
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