Validation of a Single Rest-Stress Imaging Protocol for Myocardial Perfusion Imaging

Healthy Volunteers: Inclusion Criteria: * Subjects must be ≥30 and ≤75 years of age; * Subjects must provide informed consent prior to study procedures; Exclusion Criteria: * History of CAD, including: * Prior abnormal myocardial perfusion study * History of MI * History of angina * Coronary artery obstruction \>50% on CTA and/or angiography * Left ventricular ejection fraction \<50% * Any relative or absolute contraindication to adenosine stress, including: * 2nd or 3rd degree heart block * Bradycardia (HR\<50 bpm) * Recent acute coronary syndrome (ACS) * Unstable angina * Severe heart failure (left ventricular ejection fraction \<15%) * Ventricular arrhythmia * Severe asthma and/or chronic obstructive pulmonary disease (COPD) * Baseline hypotension defined as systolic blood pressure \< 90 mmHg * Caffeine intake within 24 hours prior to imaging * Current use of theophylline, dipyridamole, or carbamazepine * Allergy or intolerance to adenosine * Positive serum and/or urine pregnancy test, or lactating, or possibility of pregnancy cannot be ruled out prior to dosing; Subjects with CAD: Inclusion criteria * Subjects must be ≥30 and ≤75 years of age; * Subjects must provide informed consent prior to study procedures; * Abnormal myocardial perfusion study within the past 12 months Exclusion criteria * Revascularization (percutaneous coronary intervention and/or coronary artery bypass) following last myocardial perfusion study * Acute coronary syndrome within 30 days * Left ventricular ejection fraction \<50% * Any relative or absolute contraindication to adenosine stress, including: * 2nd or 3rd degree heart block * Bradycardia (HR\<50 bpm) * Recent acute coronary syndrome (ACS) * Unstable angina * Severe heart failure (left ventricular ejection fraction \<15%) * Ventricular arrhythmia * Severe asthma and/or chronic obstructive pulmonary disease (COPD) * Baseline hypotension defined as systolic blood pressure \< 90 mmHg * Caffeine intake within 24 hours prior to imaging * Current use of theophylline, dipyridamole, or carbamazepine * Allergy or intolerance to adenosine * Any clinically significant acute or unstable physical or psychological disease, judged by the investigators, to be incompatible with the study; * Radiation exposure exceeds current Radiology Department guidelines (i.e. 50 mSv in the prior 12 months); * Female participants only: Positive serum and/or urine pregnancy test, or lactating, or possibility of pregnancy cannot be ruled out prior to dosing; * Inability to provide written informed consent;