This study is aimed to investigate the global transcriptome in order to determine the expression profile of messenger RNAs (mRNAs), as well as long noncoding- (lncRNAs) and micro noncoding-RNAs (miRNAs) in heart failure (HF) and in aortic stenosis (AS). The aim is to clarify their role in cardiac disease pathogenesis, as well as their potential as biomarkers. To this purpose, both tissue and blood specimens will be collected and patients will be compared to individuals not affected by cardiovascular diseases.
The investigators will collect plasma and cardiac tissue specimens from consenting subjects. The investigators will evaluate the expression of mRNAs, miRNAs and lncRNAs in patients affected by both end-stage and not end-stage HF as well as in patients affected by aortic stenosis and undergoing valve replacement and requiring cardiac myectomy. The tissue dysregulated RNAs will be next measured in plasma samples in order to clarify their potential as biomarkers.
Study Type
OBSERVATIONAL
Enrollment
500
Irccs Policlinico San Donato
San Donato Milanese, Milan, Italy
RECRUITINGOspedale San Raffaele
Milan, Italy
RECRUITINGCardiac tissutal mRNAs signatures
The mRNAs cardiac tissutal signatures of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 1 to Year 3
Cardiac tissutal miRNAs signatures
The miRNAs cardiac tissutal signatures of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 1 to Year 3
Cardiac tissutal lncRNAs signatures
The lncRNAs cardiac tissutal signatures of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 1 to Year 3
Non end-stage, end-stage heart failure, aortic stenosis mRNAs signatures in plasmas
The mRNAs dysregulated in cardiac tissues will be measured in blood plasma samples of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 3 to Year 5
Non end-stage, end-stage heart failure, aortic stenosis miRNAs signatures in plasmas
The miRNAs dysregulated in cardiac tissues will be measured in blood plasma samples of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 3 to Year 5
Non end-stage, end-stage heart failure, aortic stenosis lncRNAs signatures in plasmas
The lncRNAs dysregulated in cardiac tissues will be measured in blood plasma samples of non- and end-stage heart failure patients and of aortic stenosis patients compared to controls
Time frame: from Year 3 to Year 5
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.