In this project, PBMCs will be isolated from HDM allergic and non allergic patient and then stimulated with HDM crude extract. The expression of inflammasome and the correlation of inflammasome activation and steroid resistance will be investigated.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
As a stimulant to induce inflammasome activation
inflammasome activation by HDM
The expression of NLRP3 in PBMCs will be measured by western blot after HDM stimulation.
Time frame: 6 hours
Steroid receptor phosphorylation by HDM
The expression of steroid receptor will be detected by western blot and real-time PCR after HDM stimulation.
Time frame: 6 hours
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