NAD Supplementation Study

Maastricht University14 enrolled

Overview

This research project aims to determine whether supplementing with a mixture of the NAD+-precursors NA, NAM, and tryptophan can stimulate skeletal muscle mitochondrial function in physically compromised, elderly humans. Outcomes are related to mitochondrial function, energy metabolism, and physical function will be investigated.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

DOUBLE

Enrollment

Conditions

Aging Mitochondrial Function

Interventions

NAD-precursorsDIETARY_SUPPLEMENT

Intervention product containing the NAD+-precursors nicotinic acid, nicotinamide, and tryptophan (total of 204 NE per serving) in a whey protein source for 31 days.

ControlDIETARY_SUPPLEMENT

Control product containing amino-acid mixture resembling whey-protein not containing the NAD+-precursors nicotinic acid, nicotinamide, and tryptophan. To be taken for 31 days.

Eligibility

Sex: ALLMin age: 65 YearsMax age: 75 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Males and females * Age ≥ 65 ≤ 75 years; * BMI ≥ 20 kg/m2 ≤ 30 kg/m2; * Normal physical activity levels: maximum of 1 hour per week engagement in a structured exercise session; * SPPB score 4-9 and (pre-)frail; * Subject should be in sufficient health to participate in the experiments, to be judged by the responsible physician based on the subject's medical history. Exclusion Criteria: * Not meeting all inclusion criteria; * Smoking; * Excessive alcohol use and/or drug abuse; * Subjects with diabetes mellitus type 2; * Significant food allergies or intolerances concerning the study products; * Participation in another biomedical study within 1 month before the first study visit, possibly interfering with the study results; * Medication use known to hamper subject's safety during the study procedures; * Subjects who use selective serotonin re-uptake inhibitors (SSRI), or selective norepinephrin re-uptake inhibitors (SNRI), or monoamino oxidase inhibitors (MAO-inhibitors), or clomipramine, or St. John's wort (Hypericum perforatum); * Subjects with contra-indications for MRI; * Subjects who do not want to be informed about unexpected medical findings; * Subjects who do not want that their treating physician to be informed; * Co-morbidities to which the intervention or program the may pose as a complicating factor; * Inability to participate and/or complete the required measurements.

Locations (1)

Maastricht University

Maastricht, Limburg, Netherlands

Outcomes

Primary Outcomes

Ex vivo mitochondrial respiration

Ex vivo mitochondrial function in skeletal muscle measured by oxygen consumption in muscle fibres (muscle biopsy vastus lateralis) on lipid-derived and carbohydrate-derived substrates.