Multicenter RCT of ADV-TK Gene Therapy Improving the Outcome of Liver Transplantation for Advanced HCC

Inclusion Criteria: * 18 - 65 years of age (Male and Female). * Clinical diagnosis of advanced primary hepatocellular carcinoma who could accept liver transplantation. * Patients who had unresectable HCC with single tumor diameter \> 5 cm and ≤ 10cm; or numbers of multiple tumors \>3 and ≤ 5, and the total length of foci diameter ≤ 15 cm. * Serum AFP ≤ 10000 ng/ml before liver transplantation. * Child-pugh A-B. * No metastasis in extrahepatic main vescular and extrahepatic lymph node detected during the operation and no metastasis of other organs. * Provide written informed consent before screening. Exclusion Criteria: * Metastasis in extrahepatic organs. * HCC with Invasion in extrahepatic main vescular and extrahepatic organs. * Contraindications of operation of other organ system. * Hypersensitivity to adenovirus, GCV or similar drugs. * Serious obstacle of the mechanism of coagulation, hemorrhagic tendency, and abnormal coagulation (≥50%). * Plan to accept clinical trials of other antitumor drugs. * Immunological deficit. * HBsAg(+) and HBcAb(+) donor. * Unsuitable participate assessed by investigator.