This experiment consists on a 20-day reduction in daily step in free-living active individuals to induce physical inactivity. This will be used to test the efficacy of the anti-oxidant cocktail we aim to test as a new countermeasure in 2016 during the 60-d bed rest planed by ESA/CNES. The objective of this study is to investigate whether the cocktail of natural antioxidants XXS-2A comprising vitamin E and coupled with omega-3 helps to prevent and / or reduce the glucose intolerance and improve oxidative defenses induced by 20 days of physical inactivity through daily step reduction Although physical inactivity is reported to affect glucose tolerance within days of inactivity, we selected a period of 20 days for the effect of the cocktail to take place and assess secondary molecular mechanisms. The effect of this short period of inactivity on metabolism will moreover be boosted during the last 10 days by taking fructose, a sugar found in abundance in fruits, honey and juices, which is known to quickly trigger metabolic deregulation.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
Micronutrient cocktail supplementation with 560,7 mg/ day of polyphenols (3 pills/day), 2,1 g/day of omega-3 fatty-acids (3 pills/day), 168 mg/day of vitamin E and 80µg/day of selenium (1 pill/day)
habitual diet to be followed
Medes-Imps
Toulouse, France
Change in Lipid oxidation
change in lipid oxidation as measured by indirect calorimetry during a 4h-OGTT
Time frame: 20 days
Change in glucose concentration
Change in plasma glucose concentration during a 4h-OGTT
Time frame: 20 days
Change in insulin concentration
Change in plasma insulin concentration during a 4h-OGTT
Time frame: 20 days
Change in NEFA
Change in fasting plasma NEFA
Time frame: 20 days
Change in triglycerides
Change in fasting plasma triglycerides
Time frame: 20 days
de novo lipogenesis
incorporation of labelled fructose in VLDL-TG
Time frame: 20 days
Change in fructose oxidation
Change in 13C recovery in breath samples from ingested 1-13C fructose
Time frame: 20 days
Change in oxidative stress
Change in fasting reduced and oxidized glutathione
Time frame: 20 days
Change in fat-free mass
Change in fat-free mass as measured by DXA
Time frame: 20 days
muscle pathways involved in intertwined protein synthesis / insulin sensitivity
western blots with Vastes lateralis samples obtained from muscle biopsy
Time frame: 4h for each day of test. 12h for all the protocol
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