Advanced glycation end-products (AGEs) has been linked to ageing, and many metabolic diseases. The findings of previous experiments suggested that the extracts from polyphenol-rich bilberry might inhibit the formation of AGEs. This is a randomized double-blind trial, aims to study the effect of Vaccinium Myrtillus L. natural extracts on AGEs and human metabolism. Firstly, we will investigate the efficacy of Bilberry extracts on lowering the levels of advanced glycation end-products (AGEs). Secondly, we will conduct 16S rRNA sequencing and ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) detection to explore the role of bilberry extracts on gut microbiota as well as metabolites.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
DOUBLE
Enrollment
80
Twice a day, 2 tablets each time. Do not take any other medicine, traditional Chinese medicine, or dietary supplements.
Twice a day, 2 tablets each time. Do not take any other medicine, traditional Chinese medicine, or dietary supplements.
Huazhong University of Science and Technology
Wuhan, Hubei, China
RECRUITINGChanges in plasma AGEs levels
Using UPLC-MS/MS to detect plasma AGEs (including CML, CEL, MG-H1).
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in urinary AGEs levels
Using UPLC-MS/MS to detect urinary AGEs (including CML, CEL, MG-H1).
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in plasma sRAGE levels
sRAGE (soluble Receptor for Advanced Glycation End-products)
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in transcription levels of RAGE and AGER1
Extract and isolate peripheral blood mononuclear cells (PBMC) from participants. Using the PCR technology to detect the mRNA levels of RAGE and AGER1.
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in gut microbiota
Time frame: At 0 week (baseline), 10th week.
Changes in plasma metabolites
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in skin AGEs levels
Using AGE Reader to quickly and noninbasively measure skin AGEs by means of fluorescence techniques.
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in body weight
Time frame: At 0 week (baseline), 4th week, 10th week.
Change in body composition (body fat mass and lean mass)
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in blood lipids profile
Fasting plasma Total cholesterol, Low Density Lipoprotein, High Density Lipoprotein and triglycerides.
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in pro-inflammatory markers
Fasting plasma C-reactive protein, interleukin-6 and tumor necrosis factor-α
Time frame: At 0 week (baseline), 4th week, 10th week.
Changes in fecal short chain fatty acids (SCFA)
Time frame: At 0 week (baseline), 10th week.
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