Analysis of in Vitro Biological Reaction of Diaphragmatic Prothesis When Congenital Diaphragmatic Hernia (CDH) Repair

University Hospital, Strasbourg, France1 enrolled

Overview

In case of CDH, the incomplete development of a part of the diaphragm allows abdominal viscera to herniate into thorax cavity during fetal development and impaired lung and heart development. After birth, CDH patients who survived thanks to an active reanimation management undergo diaphragmatic repair. In case of large defect, a patch is needed to complete diaphragmatic closure. Thanks to primary cell culture from foreskin, peritoneal epithelium or cremaster dedicated to incineration investigators want to analyse biological integration of actually used prothesis and others innovations.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Congenital Diaphragmatic Repair

Interventions

Foreskin resectionPROCEDURE

During the pediatric surgery consultation, the doctor will decide between Foreskin resection, orchidopexie or inguinal hernia repair.

Eligibility

Medical Language ↔ Plain English

Inclusion Criteria: * Minor aged 0 (day of birth) to 14 years * Patient for whom an indication of posthectomy and / or cure of inguinal hernia and / or testicular ectopy is retained * Patient whose legal representatives have agreed to the use of operational waste from mesenchymal tissues (foreskin, cremaster and peritoneum) destined for incineration. * Patient affiliated to a social security scheme Exclusion Criteria: \- parents don't agree to the use of its operating waste for research purposes

Outcomes

Primary Outcomes

cell colonization

Study cell colonization

Time frame: samples are processed immediately after sampling

quality of the extracellular matrix

Quality of the extracellular matrix produced in vitro in contact with the currently used implant materials compared to biomaterials under development