The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise. In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
20
Goldring Centre for High Performance Sport
Toronto, Ontario, Canada
Muscle protein synthesis
Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-\[D5\]phenylalanine
Time frame: 4 hours
exercise performance
Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC).
Time frame: 4 days
Muscle soreness
Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm)
Time frame: 4 days
Muscle damage
Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming.
Time frame: 4 days
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