The aim the investigators study is to analyze the embryo development if oocytes of the participants are injected with sperm from a longer or shorter (2 hours) abstinence duration.
Participants undergoing an IVF treatment, are asked to respect an abstinence duration of 2-7 days. This is to obtain a sufficient number of motile sperm. A recent investigation, analyzed the quality (number and motility) of a sperm sample produced after an abstinence duration of 4 to 7 days, followed by a second sperm sample 2 hours later. The second sample (after 2 hours) was able to produce sperm cells with a higher motility. Not only has this short abstinence duration of 2 hours a positive effect on the motility, it will also reduce the sperm DNA damage, which again has a positive effect on embryo development. Because participants need gametes of high quality to obtain embryos with a high quality, the investigators want to investigate what the effect is of abstinence duration on embryo development.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
DIAGNOSTIC
Masking
NONE
Enrollment
100
Husbands will be asked to produce a second sperm sample in the masturbatorium
Universitair Ziekenhuis Brussel
Brussels, Brussels Capital, Belgium
Embryo development (blastocyst stage)
embryo development 5/6 of the preimplantation development
Time frame: 1 year
Embryo development (cleavage stage)
embryo development on day 3 of the preimplantation development
Time frame: 1 year
Fertilization
number of fertilized oocytes per number of mature oocytes
Time frame: 1 year
Utilization rate
number of embryos used for transfer + cryopreserved for the patient
Time frame: 1 year
Sperm quality
concentration and motility
Time frame: 1 year
Sperm DNA fragmentation
analysis of non-viable sperm in the two samples
Time frame: 1 year
Clinical Pregnancies
pregnancies with a fetal heart beat
Time frame: 1 year
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