FIT-AGING will determine the effect of different exercise modalities on α-Klotho protein (primary outcome) in sedentary healthy adults. FIT-AGING also investigate the physiological consequences of activating Klotho gene (secondary outcomes).
Aims: The plasma Klotho protein concentration (α-Klotho protein), which is considered a powerful biomarker of longevity, makes it an attractive target as an anti-aging therapy against functional decline, sarcopenic obesity, metabolic and cardiovascular diseases, osteoporosis, and neurodegenerative disorders. Physical exercise and physical fitness, could be α-Klotho protein activators through biochemical process. FIT-AGING will determine the effect of different exercise modalities on α-Klotho protein (primary outcome) in sedentary healthy adults. FIT-AGING also investigate the physiological consequences of activating Klotho gene (secondary outcomes). Methods: FIT-AGING will recruit 60 sedentary, healthy, adults (50% women) aged 40-65 years. Eligible participants will be randomly assigned to a non-exercise group to the usual control group (n=15), physical activity recommendation from American College of Sport Medicine, World Health Organization and American Heart Association group (n=15), High Intensity Interval Training group (n=15)) and Whole-Body Electromyostimulation group (n=15). Laboratory measures completed at baseline and 12 weeks later, include α-Klotho protein concentration, physical fitness (cardiorespiratory fitness, muscular strength), body composition, resting metabolic rate, hearth rate variability (HRV), health blood biomarkers, free-living physical activity, sleep habits reaction time, cognitive variables and health-related questionnaires. The investigators will also obtain dietary habits data and cardiovascular disease risk factors.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
74
α-Klotho protein
Plasma Klotho concentrations will be measured by ELISA using a soluble α-Klotho ELISA assay kit.
Time frame: Baseline and through study completion, an average of 12 weeks.
Physical fitness: cardiorespiratory fitness
Cardiorespiratory fitness measure through a maximum treadmill test (VO2max.).
Time frame: Baseline and through study completion, an average of 12 weeks.
Physical fitness: muscular strength.
Muscle strength measure through: Isokinetic strength, Handgrip strength and Core resistance stability.
Time frame: Baseline and post-intervention.Baseline and through study completion, an average of 12 weeks.
Body composition.
Measure by DXA.
Time frame: Baseline and through study completion, an average of 12 weeks.
Resting energy expenditure.
Evaluated by indirect calorimetry with gas analyzer breath by breath.
Time frame: Baseline and through study completion, an average of 12 weeks.
Hearth Rate Variability (HRV).
Measure by Polar RS800CX.
Time frame: Baseline and through study completion, an average of 12 weeks.
Biochemical profile.
General biochemical profile in blood samples
Time frame: Baseline and through study completion, an average of 12 weeks.
Physical activity.
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The amount of physical activity will be measured by accelerometer.
Time frame: Baseline and through study completion, an average of 12 weeks.
Reaction time.
Measure by Vienna System.
Time frame: Baseline and through study completion, an average of 12 weeks.
Cognitive test.
Auditory memory tests
Time frame: Baseline and through study completion, an average of 12 weeks.
Dietary assessment.
Measure by 24h recall and Food Frecuency Questionnaire.
Time frame: Baseline and post-Baseline and through study completion, an average of 12 weeks..
Anthopometric measures.
Measure by stadiometer and scale.
Time frame: Baseline and post-Baseline and through study completion, an average of 12 weeks..
Sleep quality.
The quality of sleep will be measured by accelerometer.
Time frame: Baseline and post-Baseline and through study completion, an average of 12 weeks..
Hormones profile
Hormones profile measure in blood samples.
Time frame: Baseline and post-Baseline and through study completion, an average of 12 weeks..