The primary objective of this study is to determine if the use of frozen surgical testicular sperm specimens for ICSI lead to different IVF outcomes when compared with the use of fresh surgical testicular sperm for ICSI.
The study seeks to isolate the effect of slow freezing and thawing of surgical sperm specimens on fertilization and embryo blastulation rates by utilizing a randomized controlled split cohort protocol including men with obstructive azoospermia undergoing surgical sperm retrieval and good-prognosis female patients.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
QUADRUPLE
Enrollment
9
half of the surgically obtained testicular sperm will be frozen for 30 minutes and subsequently thawed prior to performing ICSI
Reproductive Medicine Associates of New Jersey
Basking Ridge, New Jersey, United States
Blastulation Rate
total number of embryos reaching the blastulation stage of development
Time frame: 5-7 days post ICSI procedure
Fertilization Rate
number of 2PNs
Time frame: 1 day post ICSI
Time to Find Sperm in Fresh Specimen
total amount of time spent finding sperm in the fresh surgical testicular sperm sample
Time frame: up to 6 hours post surgery
Post-thaw recovery rate of frozen surgical sperm
analysis of sperm parameters post freezing
Time frame: 30 minutes after freezing
Aneuploidy Rate
number of chromosomally normal and abnormal embryos
Time frame: 2 weeks post trophectoderm biopsy
Implantation Rate
presence of fetal heart beat at discharge to obstetric care
Time frame: approximately 4-6 weeks post embryo transfer
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