Transfusion of Biotin-Labeled Red Blood Cells

Inclusion Criteria: * Ages 18 years and older * Weight ≥110 lbs * Hemoglobin ≥ 12.5 g/dL or hematocrit ≥ 38% for women and hemoglobin ≥ 13.0 g/dL or hematocrit ≥ 39% for men. * Meet criteria for autologous blood donation Exclusion Criteria: * Subjects with a past medical history or symptoms of blood dyscrasia, diabetes mellitus, hyperlipidemia, obstructive sleep apnea, renal disease, congestive heart failure, significant cardiac disease and / or known peripheral arterial disease. * Moderate to severe systemic hypertension (systolic blood pressure \>140 mmHg and/or diastolic blood pressure \> 95 mmHg * Systolic blood pressure \<100 mmHg and/or diastolic blood pressure \< 60 mmHg on the study day. * Positive Direct Antiglobulin Test * Consumption of biotin supplements or raw eggs within 30 days * Treatment with antibiotics in the week before initiating study participation to avoid suppression of erythropoiesis, which may accompany infection. * Blood loss in the previous 8 weeks due to epistaxis, trauma, hemoptysis, gastrointestinal bleeding, diagnostic phlebotomy (\> 30 ml) * Subjects who report tobacco or marijuana smoking within 6 months of study. * Cognitively impaired subjects, or institutionalized persons and subjects unable or unwilling to complete written informed consent * Subjects with a history of blood donation within the last 56 days. * Use of other investigational drugs/devices within 30 days of screening. * Subjects taking any medication for the treatment of diabetes including insulin * Females of childbearing potential who are pregnant or unwilling to undergo pregnancy testing; females with positive pregnancy testing on screening day will be excluded. * History of prior transfusion reaction to blood products. * Allergic reaction to biotin * Donors with naturally occurring antibodies against BioRBCs will be excluded from the study