Evaluation the Association of Microbiome Between Respiratory Tract Samples and Stool Samples in Pneumonic Patients Accompanied by Respiratory Failure

Taichung Veterans General Hospital200 enrolled

Overview

A prospective observational study. Enrolled participants admitted to ICU due to pneumonia and respiratory failure need mechanical ventilator support. Investigators collected the residual specimens, such as sputum from endotrachea aspiration, bronchoalveolar lavage fluid in those participants as the usual care in the ICU. Those residual samples were sent to extract RNA and sequence by using high-throughput sequencing (next-generation sequencing) method. Investigators will compared the microbiome feature between lower respiratory tract and stool specimens in those participants diagnosed as pneumonia with respiratory failure.

Study Type

OBSERVATIONAL

Enrollment

200

Conditions

Microbiota Pneumonia, Bacterial Respiratory Failure Lower Resp Tract Infection

Interventions

Eligibility

Sex: ALLMin age: 40 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Adults aged than 40 years old * Diagnosed as pneumonia * Respiratory failure with mechanical ventilator support Exclusion Criteria: * Less than 40 years old * Has been treated with antibiotics more than 7 days within two weeks before enrolling into study * Suspected or active pulmonary tuberculosis infection

Outcomes

Primary Outcomes

The dynamics of the airway bacterial microbiome in the lower respiratory tract specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation)

Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S rRNA sequence of bacteria in residual samples

Time frame: Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7

Secondary Outcomes

The dynamics of the gut bacterial microbiome in the stool specimens in the setting of pneumonia accompanied by respiratory failure at the onset of, and day 3 and day 7 (or before extubation)

uses high-throughput sequencing (next-generation sequencing) to sequence the 16S

Time frame: Compared the dynamic change of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7

The association of the microbiome between lower respiratory tracts and stool specimens in pneumonic patient with respiratory failure

Uses high-throughput sequencing (next-generation sequencing) to sequence the 16S

Time frame: Compared the association of mirobiome at the baseline (onset of respiratory failure within 48 hours)m day 3 and day 7