Interstitial Fluid in the Development of Cardiovascular Disease

Karolinska University Hospital100 enrolled

Overview

At a given level of serum cholesterol, patients with T2D have an increased risk of developing atherosclerosis compared with nondiabetic subjects. In a previous study we showed that the interstitial fluid-to-serum gradient of LDL and VLDL cholesterol is reduced in T2D patients compared with healthy controls. This was not found for HDL cholesterol. However, the cholesterol transporting function of HDL particles from interstitial fluid from patients with T2D were lower than in healthy controls. We hypothesize that that the apo B-containing particles in T2D patients are more susceptible to be retained or consumed in the extravascular space. We are to study if skin biopsies from T2D patients contain more cholesterol than biopsies from healthy controls. We hypothesize that samples from T2D patients are richer in cholesterol, which could explain why VLDL and LDL cholesterol are lower in relation to their plasma levels in T2D.

Study Type

OBSERVATIONAL

Enrollment

100

Conditions

Type2 Diabetes Atherosclerosis

Interventions

Collection of interstitial fluid, skin biopsies and blood

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion criteria: * Over 18 years. * For the T2D study group: Diagnosed with type 2 diabetes. Exclusion criteria: For subjects with type 2 diabetes: * Systemic inflammatory disease that requires active treatment * Thyroid disease that requires active treatment * Skin disease that requires active treatment and causes significant changes in the area of the skin that is to be investigated * Treatment with oral glucocorticoids * Levels of SR, TSH, T4 or Hemoglobin in the blood pronouncedly outside the reference range * Pregnancy For healthy controls: * Registered disease in the health declaration that requires continuous systemic treatment. Exception to the above: well controlled hypertension in which case the subject should not have treatment with more than one antihypertensive drug, the drug must not be an alpha-blocker or beta-blocker or thiazide diuretic * Skin disease that requires active treatment and causes significant changes in the area of the skin that is to be investigated * Blood pressure pronouncedly above 140/90 * Blood test results pronouncedly outside the reference range * Pregnancy

Locations (4)

Ekerö Vårdcentral

Ekerö, Ekerö Kommun, Sweden

Patient research centre, Clinic of endocrinology, Karolinska University Hospital

Huddinge, Stockholm County, Sweden

Stiftelsen Stockholms Sjukhems Husläkarmottagning

Stockholm, Sweden

GIH (gymnastik och idrottshögskolan)

Stockholm, Sweden

Outcomes

Primary Outcomes

Interstitial fluid-to-serum ratio for LDL cholesterol

Time frame: 2 hours

Cholesterol level in skin biopsies

Time frame: 30 minutes