The aim of this project is to investigate in elderly women, the effect of combined aerobic and resistive training and concomitant supplementation with omega-3 fatty acids on adipogenesis, metabolic functions and pro-inflammatory status of adipose tissue and on systemic metabolic profile.
Aging is associated with development of disturbances of lipid and carbohydrate metabolism and with systemic pro-inflammatory state similarly to the alterations observed in obese individuals. Metabolic and immune dysfunction of adipose tissue has been suggested as an important contributing trigger to this condition. EXODYA project is a clinical study in elderly individuals that aims to establish benefits of lifestyle intervention and nutritional supplementation -either alone or in combination- for the amelioration of systemic and adipose tissue metabolic and immune disturbances. Effects of 4 months´ intervention with combined aerobic plus resistive exercise training and supplementation with omega-3 polyunsaturated fatty acids on systemic metabolic profile and molecular characteristics of adipose tissue will be investigated in elderly women. Thus, a proof-of-concept will be provided for novel recommendations and guidelines for lifestyle interventions in elderly population
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
55
Combined aerobic and resistive training 3 times a week for 4 months. The strength training will be performed at moderate intensity (i.e., 50-60% of RM), and moderate volume (i.e., 2 to 3 sets per exercise). Endurance training will be performed at moderate- to high-intensity (i.e., 60-85% of VO2max) in the form of continuous and interval training (for high intensities), respectively. The training intensity will be prescribed individually on the basis of the initial exercise testing. Subjects will be thoroughly instructed on effective home-based exercise and advised to exercise in total 5 days a week.
Capsules with high content of omega 3 fatty acids (Calanus oil) will be taken every day with lunch meal. The content of omega-3 fatty acids (EPA, DHA) per day should reach 250 mg.
Third Faculty of Medicine, Charles University
Prague, Czechia
Change in adipose tissue inflammation
analysis of distribution of immune cells (flow cytometry analysis)
Time frame: through study completion, an average of 1 year
Change in adipose tissue inflammatory signalling
analysis of pro-resolving mediators (UPLC-MS/MS methodology)
Time frame: through study completion, an average of 1 year
Change in insulin sensitivity
Hyperinsulinemic izoglycemic clamp (GDR-glucose disposal rate)
Time frame: through study completion, an average of 1 year
Change in physical performance
Maximum graded exercise test on bicycle ergometer (Vo2max)
Time frame: through study completion, an average of 1 year
Change in gene expression in adipose tissue
mRNA expression of metabolic and inflammatory genes in adipose tissue
Time frame: through study completion, an average of 1 year
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Capsules with content of omega 6 fatty acids (sunflower oil) will be taken every day with lunch meal.