Evaluation of the Effect of Late Boost on HIV-uninfected Vaccines From EV06 Trial

Inclusion Criteria: 1. Participated in the EV06 trial and received all three vaccinations from EV06 trial 2. HIV uninfected adults, as confirmed by a medical history, physical exam, and laboratory tests during screening 3. Able and willing to provide written informed consent prior to screening 4. Aged at least 18 at the time of consent 5. Able and willing to complete screening (about 1 month) and available for the planned follow-up period (6 months) 6. Willing to undergo HIV testing, risk reduction counselling and receive HIV test results 7. If female of childbearing potential (unless sterilised), willing to use a non-barrier contraceptive method from screening through the end of the study. Acceptable contraceptive methods include hormonal contraceptives (injection, transdermal patch, or implant) and intrauterine device (IUD). 8. If male, willing to use male condoms and not make a woman pregnant from enrolment through the end of the study. 9. Willing to provide blood, urine and stool samples for laboratory examination Exclusion Criteria: 1. HIV-1/2 infection 2. Symptomatic and asymptomatic malaria infection (presence of malaria parasites on thick blood smear) 3. Clinically significant acute or chronic illness at the time of randomization. 4. Any clinically relevant abnormality on history or examination 5. Use of immunosuppressive medication (other than inhaled or topical immunosuppressants) 6. Receipt of immunoglobulin within past 60 days 7. Abnormal laboratory values as specified below from blood collected within 42 days prior to randomization: 1. Hematology * Haemoglobin \<9.0 g/dL or \<5.59 mmol/L * Absolute Neutrophil Count (ANC): \< 1000/mm3 or \< 1.0 x 109/L * Absolute Lymphocyte Count (ALC): ≤ 500/mm3 or ≤ 0.5 x 109/L * Platelets: ≤ 90,000 ≥ 550,000/mm3 or ≤ 90 x 109 ≥ 550 x 109/L 2. Chemistry * Creatinine: \> 1. 1 x ULN * AST: \>2.6 x ULN * ALT: \>2.6 x ULN 3. Urinalysis: abnormal dipstick confirmed by microscopy * Protein 2+ or more * Blood 2+ or more (not due to menses) 8. History or evidence of autoimmune disease. 9. Positive for Hepatitis B surface antigen (HbsAg), positive for antibodies to Hepatitis C virus (HCV) or active syphilis. 10. Receipt of blood or blood products within the previous 6 months 11. History of severe allergic reactions to any substance requiring hospitalization or emergency medical care (e.g. Steven-Johnson syndrome, bronchospasm or hypotension) 12. Prior or current participation in another investigational agent trial except to the EV06 trial 13. Current anti-tuberculosis (TB) prophylaxis or therapy 14. If female, currently pregnant (positive serum or urine pregnancy test), planning to get pregnant in the next 9 months or lactating 15. History or evidence of any systemic disease or any acute or chronic illness that, in the opinion of the investigator, may compromise the volunteer's safety or interfere with the evaluation of the safety or immunogenicity of the vaccine Volunteers will be enrolled regardless of schistosomiasis infection status. Volunteers with high S. mansoni egg count of \>2000 eggs per gram of stool at screening will be treated before vaccination. Volunteers with low S mansoni egg count of \<2000 eggs per gram of stool at screening will be asked to forgo treatment until after completion of week 24 visit of the trial. Volunteers with other helminth infections at screening will also receive treatment before vaccination.