The purpose of this study is to evaluate the establishment of immunological memory between the 3 different infant vaccination schedules with the 13-valent pneumococcal conjugate vaccine (PCV13) currently in use. We aim to determine the optimal schedule for the establishment of antigen-specific memory B-cell pool, which may serve as a correlate for longevity of immunological memory against vaccine serotypes. Moreover, we will study the transcriptome profiles expressed by peripheral lymphocytes during each immune response in an attempt to reveal immunological mechanisms beyond the antibody and circulating B cells level.
Study Type
OBSERVATIONAL
Enrollment
60
Vaccination with PCV13
'Aghia Sophia' Children's Hospital
Athens, Attica, Greece
RECRUITINGimmunoglobulin G (IgG) antibody serum titers (measured by ELISA)
measurement of IgG antibody titers in serum pre- and post- last dose of PCV13
Time frame: 28 days post last vaccine dose
switched immunoglobulin G (swIgG) B memory cell response (measured by flow cytometry)
phenotype characterisation of swIgG memory B cells pre- and post- last dose of PCV13
Time frame: 28 days post last vaccine dose
immunoglobulin M (IgM) B memory cell response (measured by flow cytometry)
phenotype characterisation of IgM memory B cells pre- and post- last dose of PCV13
Time frame: 28 days post last vaccine dose
Transcriptional profile of Peripheral Blood Mononuclear Cells (PBMC) in response to PCV13 (measured by RNA-Sequencing)
transcriptome analysis of PBMCs pre- and post last dose of PCV13 on a cohort of patients
Time frame: 7 days post last vaccine dose
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