Cartilage Adaptation and Response to Interleukins and Exercise

Bispebjerg Hospital32 enrolled

Overview

This study will examine the effect of exercise on the turnover of collagen in articular cartilage and the content of cytokines in the synovial fluid from human adults with osteoarthritis.

By using stabile isotope labelling, this study will track the formation and destination of newly formed cartilage collagen in adult humans scheduled for knee replacement surgery due to osteoarthritis. The collagen formation as well as the effect on the cytokine content of the synovial fluid will be examined both at rest and in response to 4 weeks of exercise/training. Overall the project aims to improve the basic understanding of cartilage development and function in relation to osteoarthritis prevention.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Osteo Arthritis Knee

Interventions

Exercise.BEHAVIORAL

Moderate to heavy intensity resistance exercise consisting of leg press.

Eligibility

Sex: ALLMin age: 40 YearsMax age: 90 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Primary osteoarthritis of the knee scheduled for knee replacement surgery * Must be able to perform physical exercise * Age 40-90 years old * BMI 18.5-40 kg/m\^2 * None smoker for the last 6 weeks Exclusion Criteria: * Regular intake of: * non steroid anti-inflammatory drugs (NSAID) * corticoid-steroids * disease modifying anti-rheumatic drugs (DMARD) * cytostatic incl. chemotherapy * immune suppressive medicine * Inflammatory diseases or cancer * Recent trauma to the knee (\< 3 months) * Previous knee surgery * Regular strenuous exercise ( \> 1 pr. month) * Previous exposure to tracers (e.g. from another study)

Locations (1)

Institute of Sports Medicine Copenhagen

Copenhagen, Denmark

Outcomes

Primary Outcomes

Collagen synthesis in articular cartilage

By using incorporation of stabile isotope tracers measured by gas chromatography, the concentration of newly formed collagen will be measured.

Time frame: End of study at 4 weeks

Secondary Outcomes

Cytokine and enzymes concentration in synovial fluid

By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in synovial fluid will be measured.

Time frame: End of study at 4 weeks

Cytokine and enzymes concentration in blood

By using Enzyme-Linked Immunosorbent Assay (ELISA) the concentration of cytokines in the blood will be measured.

Time frame: At the beginning and at the end of the study at 4 weeks

Gene expression

By using real time reverse transcriptase polymerase chain reaction (RT-PCR) the concentration of messenger ribonucleic acid (mRNA) in cartilage tissue will be measured.

Time frame: End of study at 4 weeks

Strength

By calculating the total volume of weight lifted (kg) the strength will be measured.

Time frame: At the beginning and at the end of the study at 4 weeks

Body mass index (BMI)

BMI measured as the total body weight divided by the height squared (kg/m\^2)

Time frame: At the beginning of the study.

Age

The age measured in years

Time frame: At the beginning of the study.

Data from ClinicalTrials.gov

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