Study of Platelets Sialylation by Flow Cytometry for the Differential Diagnosis of ICT

Nantes University Hospital40 enrolled

Overview

Idiopathic thrombocytopenic purpura (ITP) is the most frequent auto-immune cytopenia. There is no specific biological marker and the diagnosis often results from the exclusion of other differential diagnoses, notably inherited thrombocytopenia. Recent studies have reported an original platelet destruction mechanism in ITP, by antibody-mediated desialylation of membrane proteins. The detection of platelet sialylation can be readily achieved using flow cytometry. This could provide a new biomarker of ITP, useful to ascertain a diagnosis of ITP and guide towards proper patient management.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Idiopathic Thrombocytopenic Purpura

Interventions

non interventional studyOTHER

non interventional study

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Adult patients (\>18yo) with a diagnosis of ITP (primary acute, persisting or chronical) * Adult patients (\>18yo) with a diagnosis of non immunological thrombocytopenia (constitutive thrombocytopenia, myelodysplastic syndrome, chemotherapy-induced thrombocytopenia) * Adult patients (\>18 yo) without thrombocytopenia * Enrolled in a Social Security system * Having provided informed consent Exclusion Criteria: * Minor patients (\<18 yo) * Enrolled in another clinical study * Having received corticosteroids or polyvalent immunoglobulins in the past 4 * weeks or anti-platelet therapy or NSAID during the past 7 days * Having received platelet transfusion in the past fortnight * With proven iron deficiency * With drug-induced immune-allergic thrombocytopenia. * Pregnant and breastfeeding women, * guardian patients, will be excluded from this population.

Outcomes

Primary Outcomes

Assess the difference of sialylation between ITP patients and other causes of thrombocytopenia / controls

a significant decrease in platelets sialylation in ITP patients, measured in flow cytometry with fluorescent Ricinus communis agglutinin

Time frame: 18 months

Secondary Outcomes

Prognostic value and therapy

prognostic value of the level of sialylation in ITP patients regarding disease evolution and response of first line treatments and splenectomy.