Impact of Anti-HLA Donor-specific Antibodies in ABO-incompatible Kidney Transplantation

University Hospital, Toulouse58 enrolled

Overview

It was previously suggested an improvement of graft survival in ABO/HLA incompatible kidney transplantation (KT) compared with HLA (human leukocyte antigen) incompatible transplantation. Here, the investigators would analyse clinical, biological and histological results of ABO/HLA incompatible kidney transplant recipients, comparing with ABO or HLA compatible kidney transplantation.

The investigators propose to analyze the clinical, biological and histological course of kidney function, but also to investigate the T and B cells population after KT in ABO incompatible, ABO/HLA incompatible and HLA incompatible kidney transplantation (1 and 7 years posttransplantation in each groups).

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Kidney Transplant

Interventions

Blood samplingOTHER

Descriptive analysis of clinical, biological and histological of 3 arms: ABO incompatible, HLA incompatible, and ABO/HLA incompatible kidney transplantation (1 and 5 years posttransplantation in each groups)

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Patient receiving a KT and followed at least one year in Toulouse Hospital * Patient who have signed informed consent * \> 18 years old Exclusion Criteria: * Patient under protective measures * Patients treated for cancer, infectious or immune disease by molecules that could interfere with lymphocyte populations : Interleukin 6 (IL6) blockers, Programmed cell death 1(PD1) and the cytotoxic T-lymphocyte-associated antigen 4 (CTL4) blockers. * Immunosuppressive treatments withdrawal - Patient in chronic dialysis * Ongoing pregnancy or pregnancy in the past year * Past of splenectomy

Locations (1)

University Hospital

Toulouse, France

Outcomes

Primary Outcomes

Gene expression related to antibody mediated rejection

To determine expression of genes related to antibody mediated rejection (inflammation, and endothelial cell activation) by transcriptomic analyse (whole genome micro-array) on kidney biopsies

Time frame: 1 year posttransplantation

Secondary Outcomes

description of T subsets cells

to analyse of T subsets cells after Kidney transplantation in blood samples of patients by flow cytometry