Effect of Smoking on the Expression of Gelatinases in Chronic Periodontitis

Baskent University80 enrolled

Overview

This study comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the hypothesis of this study was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Smokers have greater risk for developing periodontal disease and smoking is highly correlated with greater marginal bone loss. Matrix metalloproteinases (MMP) play a major role in periodontal tissue destruction. MMP-2 and MMP-9, called gelatinases, are one of the major MMPs that plays important roles in periodontitis. To date, several studies showed the increased levels of gelatinases in periodontal disease and only a limited number of study investigated the effect of smoking on gelatinases. However, no previous study has directly investigated the possible relationship between smoking and gingival tissue gelatinases expressions (MMP-2 and MMP-9) in chronic periodontitis (CP) patients relative to periodontally healthy subjects. Therefore this study is conducted to comparatively evaluate the expressions of MMP-2 and MMP-9 in smoker vs non-smoker chronic periodontitis patients as well as periodontally healthy subjects. the of this study hypothesis was that smoking may interfere with periodontal disease by effecting MMP-2 and MMP-9 expressions.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontitis Smoking

Interventions

no interventionOTHER

this is cross-sectional study

Eligibility

Sex: ALLMin age: 16 YearsMax age: 62 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * A total of 80 subjects (35 females, 45 males, with an age range of 16-62 years) were included in this study from those patients seeking dental treatment at the Baskent University School of Dentistry between February 2010 and December 2012. Exclusion Criteria: * Patients with systemic disease; * pregnant and/or lactating women; * patients taking any medications that may influence the study.

Outcomes

Primary Outcomes

expression of gelatinases levels in gingival tissue among groups

In chronic periodontitis group, after recording the clinical parameters, gingival tissues were harvested from the deepest periodontal pocket. Whereas in periodontally healthy group, gingival tissues were collected during crown-lengthening surgery or during an extraction of a teeth for orthodontic reasons. In both groups the samples include both sulcus/pocket epithelium and underlying connective tissues. The expression of MMP-2 and MMP-9 is measured immunohistochemically

Time frame: day 1

Secondary Outcomes

difference in probing depths among groups

Clinical parameters will be recorded and difference in probing depths among the groups will be calculated

Time frame: day o

difference in probing depths among groups

Clinical parameters will be recorded and difference in clinical attahment level among the groups will be calculated

Time frame: day 0

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.