RATIONALE: Collecting and storing bone samples from patients with osteoporotic and nonosteoporotic fracture to quantitatively analyze the differentially expressed proteins to further explain the relationship between bone iron content and bone mineral density (BMD). PURPOSE: This research study is looking at changes of iron content and differentially expressed proteins in bone samples from patients with osteoporotic and nonosteoporotic. fracture.
OBJECTIVES: 1. Establish the iron content in bone specimen in patients with hip fracture. 2. Explore the correlation of BMD and differentially expressed proteins. OUTLINE: 1. Collect human bone specimens from patients with osteoporotic and nonosteoporotic Fracture. 2. Provide a repository for storage of tissue and make these specimens available for approved projects by laboratory-based investigators. 3. Collect clinical data on these patients including bone mineral density. 4. To examine the bone iron content using ICP/MS. 5. Investigate the differentially expressed proteins through quantitative analysing the global proteome of osteoporotic and nonosteoporotic fracture bone specimens.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
DIAGNOSTIC
Masking
SINGLE
Enrollment
100
Bone samples from Hip fracture were collected from the femoral head which has been replaced
Second Affiliated Hospital of Soochow University
Suzhou, Jiagnsu, China
the iron content in human bone samples
Bone samples were preprocess and decomposed by microwave.Icp-ms is used for quantitative detection of samples.
Time frame: 3 month
The value of bone mineral density
Bone mineral density was measured by double energy X-ray absorptiometer (DXA) in a week before surgery.
Time frame: one week
Quantitative Analysis of Global Proteome in bone samples
Find the differentially expressed proteins in nonosteoporotic or osteoporotic bone sample through using an integrated approach involving TMT labeling and LC-MS/MS to quantify the dynamic changes of the whole proteome of Human Species.
Time frame: 5 month
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