Analysis of Biopsies With Antibody Mediated Rejection According to the Therapy Response

Thomas Mueller50 enrolled

Overview

In adult kidney transplant recipients a therapy-responder and non-responder phenotype by antibody mediated rejection will be precisely defined according to clinical and histological characteristics such as creatinine, proteinuria and Banff-classification. Twenty-five patients will be selected each in the therapy-responder and non-responder group. Their stored Formalin Fixed Paraffin Embedded (FFPE) biopsies will be (re)-classified according to the most recent Banff classification. Using Nanostring Technology the gene transcription of therapy-responders versus non-responders will be analyzed and compared to serological and clinical parameters.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Antibody-mediated Rejection

Interventions

Transcriptome AnalysisDIAGNOSTIC_TEST

Transcriptome Analysis of Formalin Fixed Paraffin Embedded biopsies

Eligibility

Medical Language ↔ Plain English

Inclusion Criteria: * Kidney transplant patients at the University Hospital of Zurich between 01.01.2008 - 31.12.2016 with histologically suspected or confirmed ABMR * Written consent for further use of data for research purposes Exclusion Criteria: * Age at transplantation \< 18 years * Combined organ transplantation (incl. Kidney-Pancreas, Kidney-Liver, Kidney-Heart) * Incomplete laboratory and/or clinical data * Recurrence of the initial disease * Insufficient Biopsy Material for NanoString Analysis * Documented refusal of further data analysis for research purpose

Central Contacts

Thomas F. Mueller, Prof.

CONTACT

044 255 27 75 thomas.mueller@usz.ch

Onur Sazpinar