Dehydroepiandrosterone Maintain Mitochondrial Quality of Cumulus Cells in Poor Ovarian Responders

Kaohsiung Veterans General Hospital.66 enrolled

Overview

To investigate whether the DHEA supplementation could improve mitochondrial quality in poor ovarian responders

Women who underwent in vitro fertilization (IVF) treatment participated, including normal ovarian responders (NORs) and poor ovarian responders (PORs). PORs were assigned to receive DHEA supplementation or not before the IVF cycle. For all patients, cumulus cells (CCs) were obtained after oocyte retrieval. In the CCs, mRNA expression of mitochondria-related genes was measured. To compare the mRNA expression of mitochondria-related genes in the CCs among the three groups.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

TREATMENT

Masking

NONE

Enrollment

Conditions

Poor Responders Dehydroepiandrosterone

Interventions

dehydroepiandrosterone (DHEA)DIETARY_SUPPLEMENT

Participants take DHEA 90 mg daily for two months at least before in vitro fertilization cycles.

Eligibility

Sex: FEMALEMin age: 25 YearsMax age: 45 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Poor ovarian responders met the Bologna criteria, having at least two of the three following features: (1) advanced maternal age (≥ 40 years) or any other risk factor for POR, (2) a previous POR (≤ 3 oocytes with a conventional stimulation protocol), and (3) an abnormal ovarian reserve test. An abnormal ovarian reserve test was defined as antral follicle counts (AFC) \< 5 or anti-Müllerian hormone (AMH) \< 1 ng/mL in this study. * Normal ovarian responders met the following criteria: (1) AFCs ≥ 5 or AMH ≥ 1 ng/mL and (2) the number of retrieved oocytes was between 5 and 15. Exclusion Criteria: * previous oophorectomy * exposure to cytotoxic or pelvic irradiation for malignancy * positive screening for recurrent pregnancy loss (chromosome mapping, antinuclear antibodies, extractable nuclear antigens, antiphospholipid antibodies, thrombophilic screening) * any other sensitizing or ovarian stimulating therapy during the previous 3 months

Locations (1)

Kaohsiung Veterans General Hospital

Kaohsiung City, Taiwan

Outcomes

Primary Outcomes

mitochondria related genes expression

cumulus cells genes expression

Time frame: through study completion, an average of 1 year

Secondary Outcomes

oocytes

retrieved oocytes in number

Time frame: through study completion, an average of 1 year

embryos

day 3 embryos in number

Time frame: numbers will be confirmed 3 days after fertilization

pregnancy rate

clinical pregnancy rate

Time frame: pregnancy will be confirmed 4 weeks after embryo transfer

Data from ClinicalTrials.gov

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