Gluten and Amylase Trypsin Inhibitors (ATI) Free Diet

Inclusion Criteria: 1. Males and females 2-23 years of age 2. Full-sibling of an individual with a clinical diagnosis of type 1 diabetes 3. Treatment naïve of any immunomodulatory agent Exclusion Criteria: 1. Abnormal HbA1c, defined as ≥ 6% 2. Previous or current use of a gluten-free diet 3. Current diagnosis of celiac disease 4. An elevated tissue transglutaminase IgA level (\> 10 units/mL) 5. Chronic (≥ 3 months duration) gastrointestinal disease or presence of gastrointestinal symptoms within the prior 6 weeks (e.g., abdominal pain, worsening constipation, diarrhea, or recurrent nausea +/- vomiting) 6. Chronic inflammatory or autoimmune disease with the exception of well-controlled hypothyroidism or intermittent or mild persistent asthma not requiring the use of daily inhaled steroids 7. Use of any medications that affect glucose metabolism (e.g., metformin) or the immune system (e.g., use of any glucocorticoids (inhaled, intranasal, oral, intravenous) within the previous 3 months) 8. Use of any medications that could affect intestinal microbiota within the previous 3 months (e.g., antibiotics, prebiotics, or probiotics) 9. Any condition that, in the investigator's opinion, may compromise study participation or may confound the interpretation of the study results