Insulin Signaling in Skeletal Muscle

Inclusion Criteria: * Pre-diabetes as defined by an oral glucose tolerance test 2-hour level between 140 and 200 mg/dL General Inclusion Criteria: * Able to communicate meaningfully with the investigator and legally competent to provide informed written consent. * Female subjects must be non-lactating, have a negative pregnancy test, and be on acceptable birth control. Exclusion Criteria: * Oral glucose tolerance test \<140 or \>200mg/dL * Treated with any of the following medications: * Systemic glucocorticoids (more than 2 weeks), antineoplastic agents, transplant medications, fibrates, anti-retroviral medications, or thiazolidinediones within 6 months prior to screening * Start or change of hormonal replacement therapy within 3 months prior to screening * Short-acting insulin (more than15 units per day) in DMT2 subjects, indicating severe insulin deficiency within 6 months prior to screening * History or presence of any of the following conditions: * Clinically significant heart disease (New York Heart Classification greater than grade II; more than non-specific ST-T wave changes on the EKG) * Peripheral vascular disease (history of claudication) * Clinically significant pulmonary disease. * Current uncontrolled hypertension (systolic BP\>160 mmHg, diastolic BP\>100 mmHg) * History or presence of malignancy other than basal cell or squamous cell skin cancer * Autonomic neuropathy * Clinically significant hematologic disease * Any of the following abnormal laboratory values: * Hematocrit \< 35 vol% * Serum creatinine \> 1.6 mg/dl * AST, ALT or Alkaline phosphatase \> 2.5 times the upper limit of normal * PT, PTT outside the normal reference range * TSH outside the normal reference range * Triglycerides \> 400 mg/dl * Platelet count \< 50,000 * Current or history of drug abuse or alcohol abuse * Blood donation within 2 months prior to screening * Engage in exercise with moderate to hard intensity for greater than 1 hour per day for 5 or more days per week. * Diagnosed with Type 1 diabetes