The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
NONE
Enrollment
236
Single embryo culture means only one embryo is cultured in one drop, and the group culture refers to culture of 2-5 embryos in one drop. The main advantage of group culture may be secreting some paracrine cytokines by embryos and benefit each other; and the possible disadvantage of group culture is that embryo secreting of some harmful substances which accumulated to a certain extent will damage the developmental potential of embryos. The advantages and disadvantages of single embryo culture are contrary to group culture.
Reproductive and Genetic Hospital of Citic-xiangya
Changsha, Hunan, China
RECRUITINGhigh quality blastocyst formation rate
number of blastocysts with high quality divided by number of zygotes with normal fertilization
Time frame: 6 days after fertilization
embryo euploid rate
number of euploid blastocysts divided by all blastocysts detected
Time frame: about one month after blastocyst biopsy
embryo implantation rate
number of gestational sacs divided by number of embryo transferred
Time frame: about three to six months after blastocyst biopsy
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