Effect of Spinal or Sevoflurane Anesthesia on Neutrophil Activation During Knee Surgery With Pneumatic Tourniquet

University of Liege40 enrolled

Overview

Aim of study : Assessement of neutrophil activation during ischemia reperfusion associated with tourniquet use for total knee arthroplasty. This activation was compared in a group of patient scheduled for spinal anesthesia and for sevoflurane anesthesia This activation was assessed by plasma myeloperoxidase and elastase measurements in four consecutive blood samples during and after surgery (before surgical incision and 1, 3, 24 hours following tourniquet release.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

SCREENING

Masking

NONE

Enrollment

Conditions

Knee Surgery With Tourniquet

Interventions

Sevoflurane Volatile LiquidDRUG

Bupivacaine / SUFentanilDRUG

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Total knee arthroplasty under spinal or sevoflurane anesthesia Exclusion Criteria: * American Society of Anesthesiologists class 3 and 4 * vascular disease of the lower limbs * cardiac or respiratory disease * immune disorder

Outcomes

Primary Outcomes

Changes in plasmatic concentration of myeloperoxidase and elastase

Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to one hour after tourniquet release.

Time frame: Before surgical incision and one hour after tourniquet release

Changes in plasmatic concentration of myeloperoxidase and elastase

Biological measurement of changes in plasmatic concentration of myeloperoxidase and elastase from preoperative values to 3 and 24 hours after tourniquet release.

Time frame: Before surgical incision then 3 and 24 hours after tourniquet release

Secondary Outcomes

Changes in plasmatic concentration of endothelial biomarkers

Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to one hour after tourniquet release.

Time frame: Before surgical incision and one hour after tourniquet release

Changes in plasmatic concentration of endothelial biomarkers

Biological measurement of changes in plasmatic concentration of syndecan-1, soluble thrombomodulin, soluble E-selectin and VEGF from preoperative values to 3 and 24 hours after tourniquet release.

Time frame: Before surgical incision then 3 and 24 hours after tourniquet release

Data from ClinicalTrials.gov

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