The Effect of n-3 PUFA Plant Oil Intake on Human Metabolism

Huazhong University of Science and Technology150 enrolled

Overview

The findings of previous experiments suggested that polyunsaturated fatty acids(PUFAs) has been linked to anti-hyperlipidemia, and reducing the risk of cardiovascular disease.This is a randomized double-blind trial, aims to study the effect of PUFAs on blood lipids and human metabolism. Firstly, the investigators will investigate the efficacy of mixed plant oil(echium oil, camelina oil, safflower oil) and pure echium oil on improving the levels of blood lipids. Secondly, next generation sequencing (NGS), ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) and gas chromatography-mass spectrometry detection will be conducted to explore the role of PUFAs on gut microbiota as well as metabolites. Thirdly, single nucleotide polymorphism will be genotyped by Time-of-flight mass spectrometry to find the gene-environment interaction effect.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

PREVENTION

Masking

DOUBLE

Enrollment

150

Conditions

Hyperlipidemias

Interventions

The participants were asked to take corn oil capsule twice a day, 4 capsules (500mg/capsules) each time. The intervention period is about 3 months. Do not take any other medicine, traditional Chinese medicine, or dietary supplements.

Echium oilDIETARY_SUPPLEMENT

The participants were asked to take echium oil capsule twice a day, 4 capsules (500mg/capsules) each time. The intervention period is about 3 months. Do not take any other medicine, traditional Chinese medicine, or dietary supplements.

Mixed oilDIETARY_SUPPLEMENT

The participants were asked to take mixed oil(echium oil,camelina oil,safflower oil) twice a day, 4 capsules (500mg/capsules) each time. The intervention period is about 3 months. Do not take any other medicine, traditional Chinese medicine, or dietary supplements.

Eligibility

Sex: ALLMin age: 30 YearsMax age: 60 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Aged between 30-60 years of age * Marginal elevated blood lipids or hyperlipidemia\[Total cholesterol≥5.2 mmol/L(200 mg/dl) or triglyceride≥1.70 mmol/L(150mg/dl) or low density lipoprotein cholesterol≥3.4 mmol/L(130 mg/dl)\] Exclusion Criteria: * Pregnancy； * Known cardiovascular disease, diabetes, hypertension and any other chronic disease； * Known gastrointestinal disease, such as Irritable Bowel Syndrome(IBS), functional bowel disease and so on； * Acute or chronic inflammatory conditions; * Liver or kidney dysfunction; * An unwillingness to discontinue nutritional supplements； * Use of lipid-lowering, antiinflammatory, antidepressant, or blood pressure medication; * Evidence of drug or alcohol abuse； * Take antibiotics or probiotics in the last three months.

Locations (1)

Huazhong University of Science and Technology

Wuhan, Hubei, China

Outcomes

Primary Outcomes

Changes in blood lipids profile

Fasting serum total cholesterol, low density lipoprotein, high density lipoprotein and triglycerides