The aim of this research study is to characterize gut microbiota composition and activity after a daily supplementation of 4.5 g of chitin-glucan during 3 weeks. For this purpose, a monocentric longitudinal intervention study without control will be performed. Fecal microbiota composition and fecal concentrations of gut microbiota-derived metabolites (bile acids, conjugated-polyunsaturated fatty acids and short-chain fatty acids) will be measured. In addition, during ten hours following the ingestion of a test meal rich in fibers, the kinetic of production of H2, methane and volatile compounds exhaled from breath will be evaluated.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
17
4.5g per day of chitin-glucan during 3 weeks
Center of Investigation in Clinical Nutrition
Louvain-la-Neuve, Belgium
Gut microbiota composition
Illumina sequencing of 16SrDNA
Time frame: Difference between day 0 and day 21
Fecal short-chain fatty acids
Gas chromatography using Flame ionization detector (GC- FID)
Time frame: Difference between day 0 and day 21
Fecal bile acids
Gas chromatography-mass spectrometry (GC/MS)
Time frame: Difference between day 0 and day 21
Fecal conjugated-linoleic acids
Gas chromatography using Flame ionization detector (GC- FID)
Time frame: Difference between day 0 and day 21
Exhaled acetate
Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled propionate
Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled butyrate
Gas chromatography using Flame ionization detector (GC- FID) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled C2
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled CO2
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled H2S
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled O2
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled N2
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled CH4
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled CO
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled H2
Gas chromatography using Thermal Conductivity Detector (GC-TCD) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Exhaled volatile compounds
Gas chromatography using selected ion flow tube mass spectrometry - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between day 0 and day 21
Gastro-intestinal symptom - discomfort
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - nausea
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - bloating
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - flatulences
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - gastrointestinal reflux
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - cramps
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - rumbling
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - burps
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - every day during the chitin-glucan supplementation
Time frame: 3 weeks
Gastro-intestinal symptom - discomfort
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - nausea
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - bloating
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - flatulences
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - gastrointestinal reflux
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - cramps
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - rumbling
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
Gastro-intestinal symptom - burps
100 mm Visual analog scale (0 = no symptom, 100 = very severe symptom) - Area under the curve (calculated on 10 hours following high-fiber test meal ingestion)
Time frame: Difference between Day 0 and Day 21
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