To investigate gastrointestinal hormone secretions and nutrient absorption during the early meal phase we will recruit 12 healthy young men. They will be equipped with peripheral intravenous catheters for frequent blood sampling. Participants will be screened and acclimatised to the study situation on visit 1. On visit 2 and 3 participants will in random order ingest an oral glucose solution or a standardised liquid mixed meal over 2-3 min.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
12
Oral glucose tolerance test will be carried out with a glucose solution made up of 75g glucose dissolved in 300mL. To the glucose solution will be added 5g 3-OMG and 1g paracetamol 5g 3-OMG
A standardised liquid mixed meal test will be carried out using a standard liquid mixed meal (fresubin drink supplemented with 1g paracetamol).
Department of Gastrointestinal Surgery, Righospitalet
Copenhagen, Capital, Denmark
RECRUITINGPlasma GIP concentrations
Glucose-dependent insulinotropic polypeptide plasma concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma GLP-1 concentrations
Glucagon-like peptide-1 plasma concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Serum 3-OMG concentrations
Serum 3-OMG concentrations (glucose absorption marker)
Time frame: -10, -2, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30
Serum C-peptide and insulin concentrations
Glucagon-like peptide-1 plasma concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma gastrin concentrations
Plasma gastrin concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma CCK concentrations
Plasma cholecystokinin concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma PP concentrations
Plasma pancreatic polypeptide concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.
Plasma NT concentrations
Plasma Neurotensin concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma glucagon concentrations
Plasma glucagon concentrations
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Serum 3-OMG concentrations
Serum 3-OMG concentrations (glucose absorption marker)
Time frame: -40, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma acetaminophen
Plasma acetaminophen (as a measure of gastric emptying)
Time frame: -10, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma amino acids
Plasma amino acids
Time frame: -10, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min
Plasma bile acids
Plasma bile acids
Time frame: -10, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 min